Figure 7.

Expression of laminin B1 and collagen IV (α1) genes. A. Northern analysis of laminin B1 (lam Bl) and collagen IV (αl) (col IV) mRNAs is shown. Blots described in Figure 2 were hybridized to randomly primed laminin B1 or collagen IV cDNAs. Autoradiographs were exposed at −70°C with an intensifying screen for approximately 12 hours. Northern blots were performed three times with qualitatively similar results. B. Quantitative analysis of the Northern shown in A. Amounts of laminin Bl and collagen IV message are relative to amounts of actin message and are plotted as a percent of the maximal amount of expression observed in the control F9-N-4 cell line.