Table 1.
List of Cyt b gene primers designed using Primer3 online tool.
| Oligo (5′-3′) | Length | Tm (°C) | Ta (°C) | Amplicon (bps) |
|---|---|---|---|---|
| F-GCAACAAATCTACGAAAACATCAC | 24 | 57 | 55 | 226 |
| R-CGTATTGTACGTCTCGGGTG | 20 | 58 | ||
| F-GCCAACGGAGCATCACTATT | 20 | 58.3 | 55 | 298 |
| R-AGTGGAAGGTGAAGAATCGGT | 21 | 59 | ||
| F-CACGAAACTGGATCAAATAACC | 22 | 58.4 | 55 | 178 |
| R-TGGCTGGTGAGAAGTTGTCT | 20 | 58.4 | ||
| F-CCAATAACCCAAACACTATTCTGAT | 25 | 57 | 55 | 162 |
| R-AGGCTGGAGAGTGGTATGAG | 20 | 58 | ||
| F-TAACATTCCGCCCAATAACC | 20 | 59.6 | 55 | 180 |
| R-TTTGTTCTCGATTAGGCTGGA | 21 | 59.8 | ||
| F-TACAATGAATTTGAGGTGGCTTC | 23 | 60 | 55 | 200 |
| R-TTTGTACGAGAAGTATGGGTGGA | 23 | 60 | ||
| F-ACCCAAACATACTTGGAGACC | 21 | 57 | 55 | 400 |
| R-TAATGGAGTATTTTGTTCTCGATTAG | 26 | 57 | ||
| F-CACTACTCACCAAATACTATAACAGCA | 27 | 58 | 55 | 155 |
| R-CCGTAGTAAATTCCTCGTCCA | 21 | 59 |