Table 2.
List of D-loop primers designed using Primer3 online tool.
| Oligo (5′-3′) | Length | Tm (°C) | Ta (°C) | Amplicon (bps) |
|---|---|---|---|---|
| F-TCCGCTAGCATATCACCTAT | 20 | 58.5 | 55 | 247 |
| R-CCTGAAACTGGTAATGGTGT | 20 | 58.9 | ||
| F-AGGCCCATTGATAGCTGGAG | 20 | 59 | 55 | 201 |
| R-CGGGCCTGAAGACAGAAAGA | 20 | 59 | ||
| F-CCCATTGATAGCTGGAGGAC | 20 | 59 | 55 | 217 |
| R-TCGGCAGACATCAGTTATGC | 20 | 59 | ||
| F-CATTCGTTCAAGTTGCTTGC | 20 | 59 | 56 | 197 |
| R-TTGGGGTTTGACGAGGATTA | 20 | 60 | ||
| F-CATTCGTTCAAGTTGCTTGC | 20 | 59 | 57 | 462 |
| R-GTTGTGATGTCCAAGACATAAAGG | 24 | 59 | ||
| F-CCCAAAGCCGGAATTTTTA | 19 | 59 | 55 | 345 |
| R-AGCTATCAATGGGCCTGAAA | 20 | 59 |