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. 2018 Jul 6;9(52):29869–29876. doi: 10.18632/oncotarget.25581

Figure 2. Influence of TP53 hotspot mutations on hematopoiesis in colony forming unit (CFU) assays.

Figure 2

(A) CFU results of knockdown of TP53 by shRNA: knockdown of TP53 did not influence colony numbers of CD34+ cells as compared to shScr as a control. (B) CFU results of knockdown of cells transduced with wtTP53 or TP53 mutations. After irradiation there was a weaker reduction in cell colony numbers in CD34+ cells transduced with conformational mutations (R175H and R249S), whereas CD34+ cells transduced with contact mutations (R248W and R273H) did not differ from those transduced with wtTP53. (C) Erythropoiesis in CD34+ cells overexpressing wtTP53 or expressing the contact mutations R248W and R273H was significantly reduced as compared to CD34+ cells transduced with conformational mutations. (*P < 0.05; **P < 0.01; ***P < 0.001).