Figure 4.

Efficient Transcriptional Reprogramming Is Associated with Increased Chromatin Accessibility

(A) ATAC-seq reads for donor MEFs, control NT oocytes, and NT oocytes overexpressed with Toca1/Fnbp1l around the Oct4 gene locus.

(B) TSA treatment enhances transcriptional reprogramming. NT oocytes incubated with or without 50 nM TSA for 24 hr were used for qRT-PCR. Time 0 represents NT oocytes just after NT. n = 3 (time 0) or 4 (control and dnRAR). Error bars represent ± SEM. ^∗p < 0.05 by the Student’s t test.