Figure 1.

Reduced BCR Expression and Altered B Cell Compartments in Pten-Deficient Mice

(A) Immunohistochemistry staining of spleen sections from control, Pten^f/f × mb1-cre and Pten^f/f × CD19-cre mice for CD169 (green), Thy1.2 (red), and IgM (yellow) at 10× magnification. Shown pictures are representative of 2 mice per genotype.

(B) Representative flow cytometric analysis of splenocytes from mice of the indicated genotypes for expression of BCR (IgM/IgD), CD23, and CD21. Histograms compare CD5 and CD43 expression in the different B cell subpopulations (pre-gated on CD19⁺ cells): follicular B cells (Fo.B; CD21⁺/CD23⁺, blue), marginal zone B cells (MZ.B; CD21^hi/CD23^lo/−, red), and CD21^lo/CD23⁻ B cells (green). Representative data of at least 8 mice per genotype are shown. Numbers in dotplots and histograms indicate the percentages of positive cells in the respective gates.

(C) Absolute cell numbers of total B cells (gray, left), Fo.B (blue), MZ.B (red), CD21^lo/CD23⁻ (green), and B1-a B cells (white, right) in spleens from control (n = 25), Pten^f/f × mb1-cre (n = 8) and Pten^f/f × CD19-cre mice (n = 18). Central horizontal line in the box represents the median, the upper and lower boundaries of the box show the respective quartile, and whiskers indicate the range of individual data.

See also Figure S1.