Table 2.
Antibodies used in immunohistochemistry
| Antibody | Origin | Target | Dilution | Antigen retrieval | Source |
|---|---|---|---|---|---|
| PLP | Mouse (mAB, IgG2a) | Proteolipid protein | 1:1000 | St (E) | MCA839G; Serotec |
| MOG | Mouse (mAB, IgG1) | Myelin oligodendrocyte glycoprotein | 1: 1000 | St (C) | Piddlesden et al., 1993 |
| IBA-1 | Rabbit (pAB) | Ionized calcium binding adaptor molecule 1 | 1: 3000 | St (E) | 019‐19741; Wako |
| CD68 | Mouse (mAB; IgG1) | CD68 110-kD transmembrane glycoprotein in macrophages | 1 : 100 | St (E) | M0814; Dako |
| HLA-DR | Mouse(mAB; IgG1) | MHC Class II antigen | 1:100 | St (C) | M0775; Dako |
| p22phox | Rabbit (pAB) | NADPH oxidase protein | 1 : 100 | St (C) | sc-20781; Santa Cruz |
| Tmem119 | Rabbit (pAB) | Transmembrane protein 119 | 1:100 | ‐ | HPA051870; Sigma-Aldrich |
| P2ry12 | Rabbit (pAB) | Purinergic receptor | 1:2500 | St (E) | Harvard, Dr. Butovsky |
| HC10 | Mouse (mAB; IgG2a) | Heavy chain of MHC Class I | 1:2000 | St (E) | Stam et al., 1990 |
| Ferritin | Rabbit (pAB) | Iron storage protein | 1:1000 | St (E) | MO (F5012); Sigma-Aldrich |
| FTL | Rabbit (pAB) | Ferritin light chain | 1:100 | ‐ | 10727‐1-AP; Proteintech |
| CD206 | Mouse (mAB; IgG1) | Mannose receptor | 1:100 | St (E) | ab117644; Abcam |
| CD163 | Mouse (mAB; IgG1) | Haemoglobin-haptoglobin scavenger receptor | 1:1,000 | St (C) | NCL-CD163; Novocastra |
| iNOS | Rabbit (pAB) | Inducible nitric oxide synthase I | 1:200 | St (E) | PA1‐37925; Thermo Scientific |
| CD86 | Goat (pAB) | Co-stimulatory T cell signal | 1:250 | St (C) | AF-141-NA; R&D Systems |
mAB = monoclonal antibody; pAB polyclonal antibody; ST (C) = antigen retrieval with citrate buffer; ST (E) = antigen retrieval with EDTA buffer; MHC = major histocompatibility complex.