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. Author manuscript; available in PMC: 2018 Jul 24.
Published in final edited form as: Biochem J. 2015 Jun 1;468(2):293–301. doi: 10.1042/BJ20141554

Figure 3. hNUDT16 is able to hydrolase PAR- and oligo(ADP-ribosyl)ated proteins.

Figure 3

A-D Automodified PARP1 (prepared as in Fig 2) was incubated with buffer only (control) or decreasing concentrations of recombinant hNUDT9 (A), hNUDT16 (B), PARG (C) or svPDE (D) and analysed with an anti-PAR, anti-PARP1 and anti-6xHis antibodies. Note, the His6-tag in PARG recombinant protein was previously cleaved and, therefore, not visualizable by anti-6xHis antibody. E-F 1 µM of Herpetosiphon aurantiacus PARP (HA PARP) was automodified and incubated with buffer only (control) or decreasing concentrations of the recombinant hNUDT9 (E), NUDT16 (F), PARG (G) or svPDE (H), and analysed with anti-PAR and anti-6xHis antibodies. Due to the proximity of His-tagged HA PARP and His-tagged hNUDT9, hNUDT9 was indicated by an arrow in anti-6xHis western blot (lower panel).