| Primary outcomes |
| Element 2. Incubation period following sexual transmission |
Observational epidemiological studies in humans (case reports, case series, cohort studies, case–control studies, surveillance/outbreak reports) |
Observational studies that report incubation period due to sexual transmission |
| Element 3. Serial interval |
Observational epidemiological studies in humans |
Observational studies that describe sexual transmission in humans where serial interval (time between onset of symptoms between sexual partners) is reported |
| Element 4. Duration of infectiousness |
Observational epidemiological studies in humans |
Observational studies that report duration of detection of virus in semen, cervical and vaginal secretions, and saliva; diagnostic methods included reverse transcription PCR and viral culture |
| Secondary outcomes |
| Element 1. Susceptibility |
Basic research studies (in vivo/in vitro studies) |
In vivo/in vitro studies that report on the presence of virus in the female genital tract, the male genital tract, or saliva, or on sexual transmission of virus |
| Element 5. Reproduction number due to sexual transmission |
Mathematical modelling studies |
Modelling studies that report on the elements of interest |
| Element 6. Probability of transmission per sex act |
Mathematical modelling studies |
Modelling studies that report on the elements of interest |
| Element 7. Transmission rate |
Mathematical modelling studies |
Modelling studies that report on the elements of interest |
