Fig 3. Screening the MMV Pathogen Box against NanoLuc-PEST expressing axenic amastigotes, using bioluminescence.

(A) Relative bioluminescence (compared to untreated controls) of L. mexicana expressing NanoLuc-PEST was measured following treatment with compounds at two concentrations (2 and 10 μM). Reference compounds in the MMV Pathogen Box (see S2 Table) are shown as filled circles. Mean values are shown (n = 4). The dotted line indicates the level of relative bioluminescence (5% or less at 2 μM) that forms our cut off for compounds to be considered hits. (B) Represents the bottom left of Fig 3A, and shows the 23 compounds identified as hits from our screen of the MMV Pathogen Box. These compounds are depicted as the MMV Pathogen Box disease set: tuberculosis (filled triangle), schistosomiasis (open circle), onchocerciasis (open square), malaria (open triangle), kinetoplastids (filled square) and reference compounds (filled circle). The reference compounds shown here are Buparvaquone, Mebendazole and Auranofin. Mean values are shown (n = 4) from two independent experiments.