Table 1. Primer sequences for canine PD-L1, PD-L2, CD80, CD86, ACTB, RPL13A, and RPL32 genes.
Target gene | Primer name | Sequence (5'–3') | Product length (bp) | GenBank accession No. |
---|---|---|---|---|
PD-L1 | f600 | TGGCAAAACCACCATCACTA | 214 | NM001291972 |
r813 | CAGGAAAGGTCCCAGAATCA | |||
PD-L2 | F2 | AAGACCTCCCAAGGCCTCTA | 172 | XM847012 |
R2 | CATGAAGCAGCCAGTTTGAA | |||
CD80 | F | ATGGATTACACAGCGAAGTGGAGAA | 323 | AF106824 |
R | AGGCGCAGAGCCATAATCACGAT | |||
CD86 | F | ATGTATCTCAGATGCACTATGGAAC | 221 | AF106824 |
R | TTCTCTTTGCCTCTGTATAGCTCGT | |||
ACTB | F | CCGCGAGAAGATGACCCAGA | 237 | Z70044 |
R | GTGAGGATCTTCATGAGGTAGTCGG | |||
RPL13A | F | GCCGGAAGGTTGTAGTCGT | 87 | AJ388525 |
R | GGAGGAAGGCCAGGTAATTC | |||
RPL32 | F | TGGTTACAGGAGCAACAAGAA | 100 | XM848016 |
R | GCACATCAGCAGCACTTCA |