Fig 1. TbCMT1 is not required for T. brucei Lister 427 BSF proliferation in cell culture.

(A) Total RNA was purified from TbCMT1 conditional null cells cultured with (+tet) and without tetracycline for 24, 48 and 72 h. TbCMT1 expression was analysed by qRT-PCR normalised to telomerase reverse transcriptase (TERT). The delta Ct values (dCt) for nine measurements for each condition are visualised as a swarm plot to show all observations along with representations of the underlying distributions. (B) Cumulative cell counts of triplicate TbCMT1 conditional null mutant cell cultures grown with (plus) and without (minus) tetracycline. (C) Cumulative cell counts of triplicate wild type (WT) and TbCMT1 null cells cultured in parallel. For the data in panels B and C, the cell counts of three biological replicates are reported after 2, 4, 6, 8 and 10 days. The cultures were counted and diluted to 10⁵ cells/ml every two days in (B) and to 10⁴ cells/ml every two days in (C). The cell counts are reported as the log10 value of the cumulative number of parasites per ml of cell culture allowing for the aforementioned dilution factors.