Key resources table.
Reagent type | Designation | Source or reference | Identifiers | Additional |
---|---|---|---|---|
Gene (Rattus norvegicus) | VGLUT1 | Edwards lab | NM_053859.2 | |
Gene (Rattus norvegicus) | VGLUT2 | Edwards lab | NM_053427.1 | |
Gene (Rattus norvegicus) | VGLUT3 | Edwards lab | NM_153725.1 | |
Cell line (Homo sapiens) | HEK293T cells | UCSF Cell Culture Fac. | ||
Transfected construct | pEGFP-N1 | Clontech | ||
Antibody | Mouse anti-actin | Sigma | MAB1501R | (1:3000) |
Antibody | Guinea pig anti-VGLUT1 | Millipore Sigma | AB5905 | (1:2000) |
Recombinant DNA reagent | PolyJet | SignaGen | SL100688 | |
Recombinant DNA reagent | Lipofectamine 2000 | Gibco Life Technologies | ||
Software, algorithm | Prism 5.0 | GraphPad |