Table 1.
Values of hematological parameters in untreated and 4 Gy-irradiated mice
| Parameter | Untreated WT mice (n=7) |
Untreated COX-2 KO mice (n=7) |
4 Gy-irradiated WT mice (n=8) |
4 Gy-irradiated COX-2 KO mice (N=7) |
|---|---|---|---|---|
| Blood leukocyte count (× 109/l)@ | 3.01 ± 0.40 | 4.66 ± 0.70 (155%) | 0.36 ± 0.02 | 0.47 ± 0.05 (131%) |
| Blood neutrophil count (× 109/l) | 0.71 ± 0.15 | 2.33 ± 0.59 # | ND | ND |
| Blood lymphocyte count (× 109/l) | 2.24 ± 0.35 | 2.07 ± 0.11 | ND | ND |
| Blood erythrocyte count (× 1012/l)@ | 9.10 ± 0.13 | 8.89 ± 0.45 (98%) | 8.07 ± 0.14 | 7.70 ± 0.27 (95%) |
| Blood hemoglobin level (HGB) (g/l)@ | 137.3 ± 1.2 | 132.0 ± 5.7 (96%) | 123.7 ± 1.4 | 116.4 ± 3.8 (94%) |
| Hematocrit (HCT) (%)@ | 45.6 ± 0.4 | 43.9 ± 1.7 (96%) | 40.5 ± 0.6 | 38.1 ± 1.2 (94%) |
| Mean erythrocyte volume (MCV) (fl)@ | 50.9 ± 0.2 | 50.5 ±1.3 (99%) | 50.8 ± 0.2 | 49.4 ± 0.4 ** (97%) |
| Mean erythrocyte hemoglobin (MCH) (pg)@ | 15.3 ± 0.1 | 15.1 ± 0.3 (99%) | 15.5 ± 0.1 | 15.1 ± 0.1 ** (97%) |
| Mean erythrocyte hemoglobin concentration (MCHC) (g/l) | 301.2 ± 1.7 | 300.1 ± 1.8 (100%) | 305.4 ± 1.3 | 305.8 ± 0.9 (100%) |
| Blood platelet count (× 109/l) | 897.4 ± 37.7 | 1008.2 ± 57.4 (112%) | 906.2 ± 55.1 | 1038.0 ± 109.0 (114%) |
| Mean platelet volume (fl)@ | 4.53 ± 0.04 | 4.70 ± 0.02 ### (104%) | 4.51 ± 0.04 | 4.52 ± 0.04 (100%) |
| Plateletcrit (%) | 0.41 ± 0.02 | 0.47 ± 0.03 (115%) | 0.41 ± 0.02 | 0.47 ± 0.05 (115%) |
| 1Femoral bone marrow cellularity / g body weight (× 103)@ | 0.95 ± 0.02 | 1.00 ± 0.02 (105%) | 0.27 ± 0.01 | 0.21 ± 0.01 ** (78%) |
| 1GM-CFC/femur / g body weight@ | 495.9 ± 13.7 | 565.2 ± 18.78 ## (114%) | 77.0 ± 8.5 | 58.8 ± 5.7 (76%) |
| 1BFU-E/femur / g body weight@ | 505.0 ± 33.9 | 579.1 ± 20.2 (115%) | 60.1 ± 3.8 | 40.3 ± 6.3 * (67%) |
| 1Spleen weight / g body weight (g)@ | 2.62 ± 0.19 | 5.53 ± 1.10 ### (211%) | 1.59 ± 0.11 | 1.95 ± 0.17 (123%) |
The results are presented as arithmetic means ± standard errors of the means (SEM). n = numbers of mice.
- considering the differences in the average body weight between the groups of mice studied (results not shown), the values of the bone marrow parameters and that of the spleen weight were expressed as per 1 g of body weight. ND = not done (the analyzer used allows to determine numbers of neutrophils and lymphocytes only when the total leukocyte number exceeds the value of 0.5×109/l).
- value in COX-2 KO mice is statistically significantly higher (P≤0.05, P≤0.01, P≤0.001, respectively) than that in the corresponding WT counterparts.
- value in COX-2 KO mice is statistically significantly lower (P≤0.05, P≤0.01, respectively) than that in the corresponding WT counterparts.
- value of the pertinent parameter in COX-2 KO mice, expressed as per cent of that in respective WT mice, is lower in irradiated animals.