Table 2.
Feature comparison among the three 1H–15N HSQC spectra collected on a 26 kDa 15N-labeled protein.
| Type | Pulse program | Cross-peak sign | Averaged S/Na | Experimental timeb | ||
|---|---|---|---|---|---|---|
|
| ||||||
| NH | NH2 | Separation | ||||
| Standard | Bruker hsqcfpf3gpphwg | + | + | No | 32.4 | 3 h 18 min |
| Standard multiplicity-edited | hsqcfpf3gpphwg with an extra 1/1JNH period after t1 | + | − | No | 24.6 | 3 h 21 min |
| Semi-CT | The 1JNH-active version in Fig. 1A | + | − | Yes | 27.1 | 3 h 12 min |
a
A total of 137 isolated cross-peaks were picked on all spectra and peak S/N were calculated using Sparky (T.D. Goddard and D.G. Kneller, University of California, San Francisco). The averaged S/N are shown.
b
The sample is a 0.1 mM 15N-labeled retroviral capsid protein (26 kDa). All HSQC spectra were collected on a Bruker 500 MHz spectrometer equipped with a room-temperature probe.