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. Author manuscript; available in PMC: 2019 Aug 1.
Published in final edited form as: J Immunol. 2018 Jul 2;201(3):916–929. doi: 10.4049/jimmunol.1701167

FIGURE 2.

FIGURE 2

Identification of genes related to oxidative stress by the Human Oxidative Stress RT2 Profiler PCR Array. (A) PCR Arrays revealed up- and down-regulated genes in ALI cultured human airway epithelial cells treated with or without CRE (n=84). X-axis: Medium, Y-axis: CRE; Cut off: ≥ 2-fold change. (B) RT-PCR analysis of COX-2 expression in CRE-treated ALI cultured human airway epithelial cells in the presence or absence of polymyxin (n=3). (C) RT-PCR analysis of COX-2 expression in Bla g2-treated ALI cultured human airway epithelial cells (n=3). (D) Western blot analysis of COX-2 expression in CRE-treated NHBEs in the presence or absence of NAC or MitoTEMPO. (E) Quantitative data for COX-2 expression relative to expression of β-actin. NAC: N-acetyl cysteine, MT: Mito TEMPO. CRE: cockroach extract. Data are expressed as the mean ± SEM. *P<0.05, **P<0.01.