Skip to main content
. Author manuscript; available in PMC: 2019 Oct 15.
Published in final edited form as: Mol Cell Endocrinol. 2018 Feb 8;474:35–47. doi: 10.1016/j.mce.2018.02.005

Figure 3. Effect of Pyk2-deletion and estrogen stimulation on OB activity.

Figure 3

A) WT and Pyk2-KO OBs were cultured under osteogenic conditions containing ascorbic acid and β-GP, with or without 100 nM E2. ALP activity was quantified at day 14 and 28. B) WT and Pyk2-KO OBs were cultured in osteogenic media without E2 or with 1, 10 or 100 nM E2 for 28 days, and then analyzed using a quantitative mineralization assay which is based on elution of Alizarin Red S from calcium bound to the collagen matrix. All assays were performed in triplicate and error bars represent mean ± SEM. Experiments were repeated 3 or more times. Statistical significance of p<0.05 is indicated (*) within a genotype and (#) between genotypes for the matching E2-treated groups.