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. Author manuscript; available in PMC: 2018 Jul 25.
Published in final edited form as: Hum Mol Genet. 1996 Apr;5(4):517–524. doi: 10.1093/hmg/5.4.517

Figure 7.

Figure 7

RNA expression analysis with RT-PCR. (A) RT-PCR analysis of ZNF127, IPW, PAR-5, and PAR-1. The amplification was present in RT+ reactions (lane 4), but not in RT− reactions (lane 3). Lane 1, normal human brain mRNA for positive control; Lane 2, DNA derived from the translocation patient serving as positive control. It was omitted for IPW since RT-PCR spanned an intron. Lane 3, RNA derived from fibroblasts of the patient was incubated without reverse-transcriptase as control (RT−); Lane 4, RNA derived from the patient was incubated with reverse-transcriptase (RT+). (B) RT-PCR analysis for SNRPN exons proximal and distal to the translocation breakpoint. The amplification was present in RT+ reactions (lane 3), but not in RT− reactions (lane 2). Lane 1, RT-PCR product from mRNA derived from a normal human brain; Lane 2, RT-PCR product from RNA derived from the patient’s fibroblast culture without reverse-transcriptase (RT−); Lane 3, reaction product from RNA derived from the patient’s fibroblast culture with reverse-transcriptase (RT+).