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. 2018 Jul 24;8:11122. doi: 10.1038/s41598-018-29424-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Characterization of targeting and distribution of NF-SIONs in the shoulder tumor region by immunofluorescence staining, 24 hr after injection. The shown sections were stained with monoclonal antibodies (mAbs; green) against Ki-67 (A, proliferating cells), CD31 (B, endothelial cells), F4/80 (C, murine macrophages), and CD11b (D, monocytes/macrophages). Blue and red signals show the location of cell nucleus and NF-SIONs, each. White circles indicate co-localization of TAMs and NF-SIONs. Arrow; lumen of blood vessel. Scale bar: 100 μm (×20) for A and 50 μm (×40) for B, C and D.