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. 2018 Jul 24;8:11167. doi: 10.1038/s41598-018-29504-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Differentially expressed miRNAs in MSC from BALf of BOS, BOS 0p and stable LTRs. (a) Non-supervised hierarchical clustering based on the miRNA expression levels in MSC from BALf of stable LTRs, BOS 0p and BOS (n = 5, n = 2, n = 3, respectively). Cluster 3.0 and Treeview softwares were used. The dendrogram shows the relationships among miRNA expression patterns. Red colour indicates upregulated genes, black colour downregulated genes and green colour indicates no change. A total of 754 miRNAs were analysed. 3 miRNAs are up-regulated in MSC from BOS 0p samples respect to controls (fold change ≥ 1.5; p ≤ 0.05); (b) 60 miRNAs were significantly upregulated (fold change ≥ 1.5; p ≤ 0.05), when comparing the miRNA expression levels between MSC from BOS samples respect to stable LTRs (c). Data are expressed as Mean (log₂ scale) (±SD). *p ≤ 0.05; **p ≤ 0.001, two-tailed Student’s test.