Figure 7.

PsTag600‐FGF21 indirectly suppressed Th17 cell differentiation and IL17A expression. (A) Naïve CD4⁺ T cells were treated with indicated concentrations of PsTag600‐FGF21 during differentiation to Th17 cells. The supernatant IL17A levels were assayed after differentiation. (B) Naïve CD4⁺ T cells were analysed for the expression of FGF21 receptor β‐klotho. Differentiated 3T3‐L1 adipocytes were used as a positive control. (C) Coculture of naïve CD4⁺ T cells and differentiated 3T3‐L1 adipocytes /AML12 cells/C2C12 myoblasts with/without PsTag600‐FGF21 (3.7 μg·mL⁻¹) during differentiation to Th17 cells. Flow cytometry analysis and quantification of IL17A expression on CD4⁺ T cells after differentiation. (D) Co‐culture of differentiated 3T3‐L1 adipocytes and naïve CD4⁺ T cells were pretreated with GW9662 (10.0 μmol·L⁻¹) for 1 h, followed by incubation with PsTag600‐FGF21 (3.7 μg·mL⁻¹) during differentiation to Th17 cells. The supernatant IL17A levels were assayed after differentiation. Data shown are means ± SD (n = 6 per group). *P < 0.05, significantly different as indicated.