Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Jul 24;9:2895. doi: 10.1038/s41467-018-05260-y

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 2 — Direct visualization of Abp1 binding to actin filaments. a Domain layouts of yeast Abp1 and Abp1-SNAP proteins. b Representative step photobleaching of a streptavidin-anchored Abp1-SNAP-biotin-649 molecule. Plot shows fluorescence intensity over time. Inset shows montage of images (1.6 × 1.6 µm) for the same spot analyzed in the plot. c Fraction of Abp1-SNAP-biotin-649 molecules (introduced at 10 pM during anchoring) that photobleached in one vs. two steps (>2 photobleaching steps was never observed) from analysis as in (b). Mean fractions from 4 separate trials with total of n = 448 spots. Error bars, s.e.m. d Montage of labeled Abp1-SNAP-649 (magenta) binding to anchored OG-labeled actin filaments (cyan) over time (arrow, 50 s intervals). Scale bar, 5 µm. e Concentration-dependent Abp1-SNAP-649 binding to OG-labeled actin filaments as indicated by increasing fluorescence intensity on filaments (normalized to filament length). Data averaged from n = 10 filaments per concentration of Abp1-SNAP-649. Error bars, s.d. f Distribution of Abp1-SNAP-649 fluorescence on an actin filament at different time points, showing that intensity increases all along the length of the filament over time. g Two representative montages (0.25 s intervals) showing dynamic interactions (yellow arrows) of Abp1-SNAP-649 (magenta) molecules on tethered OG-labeled actin filaments (cyan). h Distribution of lifetimes of Abp1-SNAP-649 molecules on OG-labeled actin filaments analyzed from n = 200 binding events. Average lifetime, <t>, with s.e.m.