Figure 3.

Silver could directly disrupt bacterial Trx system. E. coli DHB4 cells grown to OD_600nm of 0.4 were incubated with antibiotics and Ag⁺ in combinations for 10 min, and ebselen and Ag⁺ in combination was used as the positive control. (A) Trx and (B) TrxR activities were detected by using DTNB reduction assay in the presence of TrxR or Trx in E. coli DHB4 cells extracts. (C) E. coli DHB4 cells grown to OD_{600 nm} of 0.4 were incubated with antibiotics and Ag⁺ in combinations for 60 min, and ebselen and Ag⁺ in combination was used as the positive control. E. coli DHB4 cells extracts were precipitated by 5% TCA, and further alkylated with 15 mM AMS and the redox state of Trx1 was analyzed by redox Western blot. The mean ± s. d. of 3 independent experiments was calculated. The t-test significances were calculated between control and rest groups, and *p < 0.05, **p < 0.01, ***p < 0.001.