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. 2018 Jul 24;8:11131. doi: 10.1038/s41598-018-29313-w

Figure 5.

Figure 5

Silver and antibiotics in combinations could not directly disrupt the bacterial GSH system. E. coli DHB4 cells grown to OD600 nm of 0.4 were incubated with antibiotics and Ag+ in combinations for 60 min, and ebselen and Ag+ in combination was used as the positive control. (A) Total amounts of GSH were detected by GR-coupled DTNB reduction assay in E. coli DHB4 cells extracts. (B) Changes in protein S-glutathionylation in E. coli. The mean ± s. d. of 3 independent experiments was depicted. The t-test significances were calculated between control and rest groups, and *p < 0.05, **p < 0.01, ***p < 0.001.