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. 2018 Jul 24;8:11142. doi: 10.1038/s41598-018-29465-9

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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PEDF regulation of the expression of endothelial cell markers and surface receptors. (A) The effect of PEDF (100 ng/ml) treatment of MDMEC on the relative mRNA expression of CD31, VEGFR2, VCAM-1, E-Selectin, and P-Selectin as determined by semi-quantitative real time PCR 4 hours after PEDF treatment. The average value for untreated cells (control) was set at 1. Mean ± SD; n = 3. **p < 0.001, ***p < 0.001, Student’s t-tests was used for statistical analysis. (B,C) Representative flow cytometry analysis of endothelial cell markers and surface receptors 24 hours after PEDF treatment. Similar results were obtained in another experiment.