Figure 5.

Toxicity of peptides Ctn[15–34] E₁₀-Ctn[15–34] and GS₄-Ctn[15–34]. (a) In vitro neutral red uptake assay for Caco-2 cells. Viability (fraction of viable cells) as compared to untreated Caco-2 cells as a function of peptide concentration. Diamonds: filled squares: PBS control, open squares: Ctn[15–34] open diamonds: E₁₀-Ctn[15–34] open triangles: (GS)₄-Ctn[15–34]. Error bars are standard deviations of replicate measurements. (b) In vivo toxicity of the peptides for Galleria mellonella larvae. Percentage surviving larvae as a function of time. Filled squares: H₂O control, filled triangles: no treatment control, open triangles: Ctn[15–34] open diamonds: E₁₀-Ctn[15–34] open squares: (GS)₄-Ctn[15–34]. For each peptide a group of n = 15 larvae were used, error bars are standard deviations within the group.