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. 2018 Jul 24;8:11129. doi: 10.1038/s41598-018-29410-w

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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DNFB-induced AD-like lesions in BALB/c mice and MT dynamics. (A) Clinical picture of DNFB-induced AD-like lesions. (B) Evaluation of DNFB-induced AD-like lesions. (a) Scores of the lesions. Skin lesions of erythema, edema, exfoliation, and scaling were scored as 0 (none), 1 (mild), 2 (moderate), and 3 (severe)^17,18. *P < 0.01 versus model group on Day 1. ^#P < 0.01 versus model group on Day 3. (b) The thickness of the skin. Skin thickness was measured three times with three different sites of the back. The average values were presented. *P < 0.01 versus control group. ^#P < 0.01 versus model group on Day 1. (c) The weight of the skin. Dorsal skin measuring 6 mm × 6 mm at a fixed position across all the mice was sheared without subcutaneous fat. *P < 0.001 versus control group. (C) (a,b) The expression of the mRNA level of MT-1 and MT-2 in the skin tissue. The primary value of Ct was transferred into 2^−ΔΔCt. *P < 0.01 versus control group. (c) The expression of the protein level of MT-1 in skin tissue.