Fig. 4.

Subcellular localization of PTEN Q17E. a COS-7 cells were transfected with pRK5 plasmids encoding the indicated PTEN variants, and cells were analyzed by immunofluorescence using anti-PTEN antibody. In the top panels, representative images are shown. In the bottom panels, quantification of percentages of cells with nuclear (N), cytoplasmic (C), or nuclear/cytoplasmic (N/C) localization is shown. b COS-7 cells were transfected with plasmids encoding the indicated PTEN-GFP variants (C-terminal tagging) (WT, wild type) and analyzed by fluorescence microscopy. In the top panels, representative images are shown. In the bottom panels, quantification of percentages of cells with nuclear (N), cytoplasmic (C), or nuclear/cytoplasmic (N/C) localization is shown