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. 2018 Jul 18;9:1604. doi: 10.3389/fmicb.2018.01604

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Copyright © 2018 Bell, Garland and Alford.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The relationships between (A) the number of totally inhibitory bacterial isolates identified and sequenced and the expected number of totally inhibitory OTUs (EstimateS; Colwell, 2013), and (B) the total number of isolates obtained from each individual frog and the number of totally inhibitory OTUs detected. The size of circles corresponds to the number of frogs at each combination of number of isolates and number of OTUs, with the smallest indicating one individual and the largest indicating eight. The best fit accumulation curve is a power curve fitted to the number of OTUs plus 1 (y = 0.717x^0.449 with R² = 0.505, p = 0.001).