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. 2018 Jul;155(1):130–143.e15. doi: 10.1053/j.gastro.2018.03.040

Figure 4.

Figure 4

Loss of DGAT1 results in decreased lipid droplet formation in fibroblasts. (A) Left: Representative contour plots for forward (FSC-A) and SSC-A of patient 3 and 2 control fibroblasts with and without OA addition. Right: Mean SSC-A of 5 technical replicates. (B) Left: Representative histogram of Nile Red mean fluorescence intensity (MFI) of patients 3 and 2 control fibroblasts with and without OA addition. Right: MFI of 5 technical replicates. (C) Western blot showing retroviral-mediated delivery of exogenous DGAT1 and DGAT2 on patient 3 fibroblasts. (D) Mean SSC-A and (E) MFI of Nile Red staining on patient 3 fibroblasts reconstituted with empty vector (EV), WT DGAT1, or WT DGAT2. Statistical analysis was done using 2-way analysis of variance with Tukey’s multiple comparison test. Mean ± SD is indicated; **P< .01 or ****P < .0001.