Figure 4.

CRY is essential for a fast re-entrainment of the activity rhythms to delays of 12:12 red-dark cycles (RD). (A) Average actograms of WT flies (n = 53), cry⁰¹ mutants (n = 60; both in the WT “Lindelbach” genetic background) and cry⁰¹ mutants with cry rescued in photoreceptors R1–6 (n = 48; ninaE-gal4;uas-cry;cry⁰¹) plus relevant controls (n = 64; uas-cry;cry⁰¹). Bars on top represent the RD cycle (R = red, D = black) that is phase-delayed by 8 h two times during the recording. The daily median of activity is indicated, as judgment of rhythm phase. (B) Quantification of the phase shifts. The left diagram shows the number of days flies of different genotypes needed to re-entrain to the phase-delayed RD cycle (means ± SEM). The right diagram depicts the number of hours the flies had phase-shifted at day 3 (means ± SEM). Numbers in columns indicate the number of tested flies. Strains that are significantly different from each other (p < 0.05) are marked by different letters.