Table 1.
Substrate scope analysis of Type I BVMOs.
| Mix | Substrate | CAS number | Sle_13190 | Sle_62070 | |
|---|---|---|---|---|---|
| 1 | 2-hexylcyclopentanone | 13074 | 65-2 | +++++ | ++++ |
| 1 | 3-methyl-2,4-pentanedione | 815 | 57-6 | - | - |
| 1 | benzylphenyl sulfide | 831 | 91-4 | - | +++ |
| 1 | Cycloundecanone | 878 | 13-7 | - | - |
| 1 | Indole | 120 | 72-9 | - | - |
| 1 | Phenylacetone | 103 | 79-7 | +++++ | +++++ |
| 2 | 2-propylcyclohexanone | 94 | 65-5 | +++++ | ++ |
| 2 | 3-octanone | 106 | 68-3 | - | ++ |
| 2 | bicyclo[3.2.0]hept-2-en-6-one | 13173 | 09-6 | +++++ | +++++ |
| 2 | Cyclododecanone | 830 | 13-7 | - | - |
| 2 | Cyclopentanone | 120 | 92-3 | - | - |
| 2 | methyl-p-tolyl sulfide | 1519 | 39-7 | ++++ | ++++ |
| 3 | 2-phenylcyclohexanone | 1444 | 65-1 | ++++ | +++++ |
| 3 | androsta-1,4-diene-3,17-dione | 897 | 06-3 | - | - |
| 3 | Cyclopentadecanone | 502 | 72-7 | + | + |
| 3 | Nicotin | 54 | 11-5 | - | - |
| 3 | Vanillylaceton | 122 | 48-5 | - | - |
| 4 | 4-hydroxyacetophenone | 99 | 93-4 | - | - |
| 4 | 4-phenylcyclohexanone | 4894 | 75-1 | +++++ | +++++ |
| 4 | androst-4-ene-3,17-dione | 63 | 05-8 | ++++ | ++ |
| 5 | 4-octanone | 589 | 63-9 | - | + |
| 5 | Acetophenone | 98 | 86-2 | - | - |
| 5 | Cyclohexanone | 108 | 94-1 | +++ | + |
| 5 | Pregnenolone | 145 | 13-1 | - | - |
| 5 | Thioanisole | 100 | 68-5 | +++ | ++++ |
| 6 | 2-octanone | 111 | 13-7 | + | - |
| 6 | 4-methylcyclohexanone | 589 | 92-4 | ++ | +++ |
| 6 | Benzoin | 119 | 53-9 | ++ | +++++ |
| 6 | Cyclooctanone | 502 | 49-8 | - | - |
| 6 | Stanolone | 521 | 18-6 | ++ | +++++ |
The 30 compounds tested in the substrate scope analysis are shown with their respective CAS number. Conversion was determined semi-quantitatively for each substrate by analysis of the peak areas in the GC chromatograms normalized by the compound(s) of the mixture that were not accepted by the enzyme. The results are categorized by the observed degree of conversion as 100–81%, +++++; 80–61%; ++++; 60–41%, +++; 40–21%, ++; <20, +; or <1%, -.