Figure 2.

Addition of Lipids to Delipidated UapAG411V_Δ1-11 Reforms the Dimer

(A) Mass spectra showing the effects on oligomerization of adding PC (34:1, upper), PI (34:1, middle), and PE (34:1, lower) to delipidated UapAG411V_Δ1-11. Lipid binding peaks are highlighted. Lipids were added to delipidated UapAG411V_Δ1-11 at a ratio of 100:1 lipid/UapA.

(B) Relative abundances of monomer and dimer species in the presence of PC (upper), PI (middle), and PE (lower) were quantified using UniDec software (Marty et al., 2015). The mass spectra are representative of three independent experiments carried out under identical conditions. The relative abundance data are the average ± SD (n = 3) and the average values are given above each bar.