Figure 4.
mTORC2 and mTORC1 are involved in monocyte adhesion induced by LPS to ECV cells under static and flow conditions. (A) The experimental design is described in detail in the Methods section. Effects of 10−9 M rapamycin and 10−6 M WYE-354 on THP-1 cell adhesion under static (B,C) and flow conditions (D,E). (B,D) Images are representative of the experiments. a, control; b, LPS; c, LPS + rapamycin; d, LPS + WYE-354. Red arrows indicate adhered monocytes. Scale bar, 50 μm. (C,E) Quantification analyses. The results are presented as means ± SE. *p < 0.05 vs. LPS.