Figure 6.

LPS induces the mTORC1/S6K pathway in monocytes. The effects of (A) LPS on mTOR and S6K phosphorylation at residues S2448 and T389, respectively (n = 3). (B) The effect of 10⁻⁶ M calphostin C on LPS-induced S6K phosphorylation (n = 3). The levels of phosphorylation were determined by the relationship between the optical density of specific phosphorylated residues and total fractions. The result is expressed in fold change in relation to an untreated control (bar graph). mTOR, mammalian target of rapamycin; S6K, S6 protein kinase; Akt/PKB, protein kinase B; Calph C, calphostin C. Images are representative of three independent experiments. The results are presented as means ± SE. *p < 0.05 vs. unstimulated non-adhered cells (control). #p < 0.05 vs. LPS.