Table 4.
Short list of the taxon-specific reference assays for the most commonly transformed crops that are proposed as best candidates for the relative quantification of GM events. The number of estimated targets for each assay, the presence of mismatches in the primers and probe annealing regions of the secondary targets, and the recommendation for GMO analysis are presented in the table.
| Crop | Assay name | Estimated number of DNA targets | Presence of mismatches in secondary primers and probe annealing regions | Recommended for GMO analysis |
|---|---|---|---|---|
| soybean |
Le1 A | 1 | – | Yes |
| Le1 B |
1 |
– |
Yes |
|
| maize |
hmg | 1 | – | Yes |
| ZmAdh1 | 1 | – | Yes, although one reaction mix for hmg was reportedly more stable than the assay for ZmAdh1 in terms of quantification performance (Paternò et al., 2009) | |
| aldolase |
2 |
Yes, one mismatch in the reverse primer and one in the probe |
No, secondary target with mismatches in the primers and probe annealing region |
|
| cotton |
acp1 | 2 | Yes, two mismatches in the primer forward, one in the primer reverse, and one in the probe | No, secondary target with mismatches in the primers and probe annealing region |
| AdhC | 1 | – | Yes | |
| SAH7 |
2 |
No |
The assay for AdhC should be preferred, but SAH7 can also be used because primers and probe anneal without mismatches to both target DNAs |
|
| oilseed rape | cruA | 2 | Yes, position and number of mismatches not clear | No, secondary target with mismatches in the primers and probe annealing region |
| ccf | 4 | Yes, position and number of mismatches not clear | No, secondary target with mismatches in the primers and probe annealing region | |
| FatA(A) | 1 | – | Yes | |
| FatA | 2 | Yes, position and number of mismatches not clear | No, secondary target with mismatches in the primers and probe annealing region |