Table 2. Optimisation of the disulfide rebridging of trastuzumab 18 with diBrPD 17e.

	Protocol			Temperature/°C				Equivalents of diBrPD x
	1	2	3	4	8	25	37	15	20	30	40	50
% Half antibody a	23	20	8	3.5	4.8	14.1	18.5	6.0	4.8	4.0	0 c	1.7 c
PDAR b	4.1	4.1	3.9	4.2	3.8	3.9	4.2	3.9	3.9	3.9	3.6	3.3

^aCalculated from densitometry traces in Fig. 4 using Image J. See ESI for details.

^bPDAR was calculated using UV-vis spectrophotometry. See ESI Fig. S19–S21.

^cLow PDAR values indicate these values may not be representative. See discussion above.