Fig. 3.

Production of C₅, C₁₀, and C₁₅ isoprenoid alcohols and their derivatives in engineered E. coli. a Production and fractional compositions (%) of IPP/DMAPP (C₅)- and FPP (C₁₅)-derived isoprenoids from strains NA-MBF1.0 and NAK-MBF1.0. Strains NA-MBF1.0 and NAK-MBF1.0 are E. coli DH5α strains harboring pSNA and pSNAK, respectively, along with pT-MBF1.0. b Production and fractional compositions (%) of IPP/DMAPP (C₅)-, GPP (C₁₀)-, and FPP (C₁₅)-derived isoprenoids from strains NA-MBF2.0 and NAK-MBF2.0. Strains NA-MBF2.0 and NAK-MBF2.0 were transformed with pSNA and pSNAK, respectively, along with pT-MBF2.0. Cultures were incubated at 30 °C for 48 h in 2YT medium containing 2.0% (v/v) glycerol and were initially induced with 0.5 mM IPTG. The error bars represent the range from three independent experiments