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. 2018 Jul 24;11:210. doi: 10.1186/s13068-018-1210-0

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — An optimized synthetic pathway for the production of C₅ and C₁₅ isoprenoid alcohols and their derivatives. Production of IPP/DMAPP (C₅)- and FPP (C₁₅)-derived isoprenoids from strains NA-MBF1.1, NA-MBF1.2, NA-MBF1.1a, and NA-MBF1.2a harboring pT-MBF1.1, pT-MBF1.2, pT-MBF1.1a, and pT-MBF1.2a, respectively, in addition to pSNA. Cultures were incubated at 30 °C for 48 h in 2YT medium containing 2.0% (v/v) glycerol and were initially induced with 0.5 mM IPTG. The error bars represent the range from three independent experiments. The two dotted lines represent the production of C₅ products (black) and C₁₅ products (orange) from strain NA-MBF1.0