Figure 7.

Lipid nanoparticle and polyplex delivery of CRISPR. (A) Combining bioreducible lipid nanoparticles and anionic Cas9:sgRNA complexes drives the electrostatic self-assembly of nanoparticles (see TEM micrograph of 3-O14B/Cas9:sgRNA nanoparticles) for potent protein delivery and genome editing. Adapted with permission from Wang et al. (2016). Copyright 2017 National Academy of Sciences. (B) Microinjection of PEI with Cas9- and sgRNA-encoding plasmid DNA into mouse brain directed against the Ptch1 locus to generate a malignant brain tumor model. Compare the wild type and Trp53± H&E stained micrographs (arrows indicate small lesions encompassing only one cerebellar folium) with the tumor from the Trp53^−/− condition (MB = medulloblastoma). Adapted from Zuckermann et al. (2015). Copyright 2015 The Authors (CC BY license).