Skip to main content
. 2017 Dec 20;25(1):112–121. doi: 10.1080/10717544.2017.1417511

Figure 5.

Figure 5.

Migration inhibition effect of ADH-1-modified liposomes (A-LP/PTX) on MCF7 PTX-R cells. (A) Wound scratch assays were performed with a uniform scratch using a 200-μL pipette tip. Then, the cells were washed with culture media to remove any free-floating cells and debris and incubated in culture medium containing LP/PTX, A-LP/PTX, free ADH-1 (2 μM) before treated with PTX (A/PTX) or free ADH-1 (2 μM) alone for 24 h and observed at ×5 magnification with inverted microscope. (B) Relative motility was calculated using 10 randomly chosen distances across the wound at 0 h and 24 h. **p < .01, versus LP/PTX. (C) Optical images of cells (white arrows) on the bottom surface of the Transwell inserts after treatment with LP/PTX, A-LP/PTX, free ADH-1 (2 μM) before treated with PTX (A/PTX) or free ADH-1 (2 μM) alone for 24 h. (D) The inhibition rate was calculated by counting cells that migrated through polycarbonate membranes of the inserts. *p < .05, versus LP/PTX.