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The LA-nBSA was incepted by the cells through macropinocytosis-LEs-lysosome pathway. (A) DsRed-Rab34 transfected MCF-7 cells were then treated with 1 mg mL−1 FITC-labeled nBSA and FITC-labeled LA-nBSA for 20 h, (B) DsRed-Rab34 and EGFP-Rab7 co-transfected MCF-7 cells were then treated with 1 mg mL−1 nBSA and LA-nBSA for 20 h. Scale bars: 10 μm. (C) Schematic representation of the pathways that nBSA and LA-nBSA enter the cells.
