Figure 4.

LA-nBSA induces autophagy and is sequestered by the autophagosomes. (A) Representative images and quantification of MCF-7 cells with EGFP-LC3 vesicles (autophagosomes). EGFP-LC3-transfected cells were treated with 1 mg mL⁻¹ nBSA and LA-nBSA for 20 h. Scale bars: 10 μm. LC3I/II protein levels were analyzed by western blotting in the MCF-7 cells treated in (A). (B) DsRed-LC3 transfected MCF-7 cells and then treated with 1 mg mL⁻¹ FITC-labeled nBSA and FITC-labeled LA-nBSA for 20 h; (C) MCF-7 cells were treated with 1 mg mL⁻¹ FITC-labeled nBSA and FITC-LA-nBSA for 20 h, respectively, and then P62 was detected with primary antibody against P62. Scale bars: 10 μm.