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. 2018 Mar 8;25(1):738–749. doi: 10.1080/10717544.2018.1446476

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© 2018 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Cellular uptake and antiproliferation studies in MDA-MB-231 cells. (A) Cellular accumulated amounts of CUR quantitatively analyzed by flow cytometry. CUR or Lys-αTS/CUR NA (10 μg/mL CUR concentration) was incubated for 2 and 6 h. Black, green, and red colors indicate control, CUR, and Lys-αTS/CUR NA group, respectively. Each point represents the mean ± SD (n = 3). ###p < .001, compared with CUR group. (B) Intracellular distribution of NPs monitored by CLSM imaging. CUR or Lys-αTS/CUR NA (10 μg/mL CUR concentration) was incubated for 2 and 6 h. Green and blue colors indicate CUR and DAPI, respectively. The length of the scale bar in the image is 20 μm. (C) Antiproliferation assay in MDA-MB-231 cells. Cell viability (%) values of Lys, Lys-αTS, CUR, and Lys-αTS/CUR NA, according to CUR concentrations, are presented after 48 and 72 h incubation. Each point represents the mean ± SD (n = 3). #p < .05, compared with CUR group. ###p < .001, compared with CUR group.