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. 2018 Feb 27;25(1):668–678. doi: 10.1080/10717544.2018.1444683

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© 2018 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Tumor necrosis three days after treatment. Representative H&E stained tumor sections from the (A) control group; (B) L-BEZ group; (C) IRE group; and (D) IRE + L-BEZ group. The tumors treated with IRE and IRE + L-BEZ had higher mean necrosis percentages than did the tumors in the other groups. The insets in (C) and (D) illustrate that live tumor cells were found within the margin of the tumors, while the necrotic cells were found in the center of the electroporated area. No living cells were observed in the IRE + L-BEZ-treated tumors. Tumor size at the start of the treatment: IRE, 11.6 × 10.6 mmand IRE + L-BEZ, 11.3 × 11.4 mm. (E) Comparison of percentage of necrosis by H&E staining (one-way ANOVA followed by Holm-Šidák post-hoc analysis, p values between: CTL and LBEZ, .094; CTL and IRE, <.001; CTL and IRE + LBEZ, <.001; LBEZ and IRE, <.001; LBEZ and IRE + LBEZ, <.001; and IRE and IRE + LBEZ, .185).