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EGFR过表达和EGFR突变的H1299细胞系的构建检测。A：转染后，H1299细胞呈现GFP阳性（×100）；B、C：EGFR mRNA和蛋白表达情况；与阴性对照组或空白对照组相比，^***P < 0.001。NC：阴性对照（EGFR空载体）；BC：空白对照（EGFR野生型）；790M：EGFR-T790M突变；LR: EGFR-L858R突变；OE：EGFR过表达；DEL：EGFR-E746-A750缺失突变。

Construction of EGFR over-expression and mutated H1299 cells. A: After transfection, H1299 cells showed GFP signal; B, C: Expression of EGFR mRNA and protein. Compared with negative control or blank control group, ^***P < 0.001. NC: negative control (empty vector); BC: blank control (EGFR-wild type); 790M: EGFRT790M; LR: EGFR-L858R; OE: EGFR over-expression; DEL: EGFR-E746-A750del; EGFR: epidermal growth factor receptor.