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Nuclear translocation of Nrf2 in hepatocytes as demonstrated by immunofluorescent multi-staining. Immunoreactivity and colocalization of Nrf2 protein were analyzed in cells pretreated with t-BHQ, MIG, or different concentrations of LE for 24 h and then exposed to 80 nM TP for 18 h. Blue indicates nuclei (DAPI staining), red indicates cytoskeleton (phalloidin), and green indicates Nrf2. Original magnification 400× Bar =20 μm.
