Table 2.
m-Values from urea and thermal denaturation experiments with WT ACBP in 50 mM Potassium Phosphate pH 7 buffer.
| Experiment | Conditions* |
m** (kcal mol−1 M−1) |
|---|---|---|
| Urea Melt | 4.9 M Urea, 25°C, 0 pN | 1.28 ± 0.02 |
| Force | ||
| Thermal Melt | 0–1 M Urea, 52.7°C, 0 pN | 1.09 ± 0.01 |
| Force | ||
| Thermal Melt | 1–2 M Urea, 48.1°C, 0 pN | 1.13 ± 0.01 |
| Force | ||
| Thermal Melt | 2–3 M Urea, 43.1°C, 0 pN | 1.13 ± 0.01 |
| Force | ||
| Thermal Melt | 3–4 M Urea, 37.3°C, 0 pN | 1.24 ± 0.01 |
| Force | ||
| Thermal Melt | 4–5 M Urea, 30.1°C, 0 pN | 1.27 ± 0.02 |
| Force |
*
For urea melt, the reported urea concentration is the Cm
**
Uncertainties determined from standard error of four independent measurements