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. 2018 Jan 15;25(1):256–266. doi: 10.1080/10717544.2018.1425777

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© 2018 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — (A) Penetration of NBD-DPPE-labeled lip, CA IX-lip, CPP33-lip, and dl-lip throughout A549 3 D tumor spheroids by confocal laser scanning microscope using Z-stacking imaging with 20 μm intervals (scale bars, 250 μm). (B) Representative brightfield images and (C) Cell viability of spheroids after treated with TPL-lip, CA IX-TPL-lip, CPP33-TPL-lip and dl-TPL-lip for 4 days with TPL concentration of 100 μM (mean ± SD, n = 10). Scale bars, 750 μm; ***, p < .001.