Figure 3.

Assessment of HC survival upon gentamycin treatment with or without compound A or B. (A) The schematic diagram of the experiment. (B) Confocal images of the apical, middle, and basal turns in the control (untreated, a1–c1) and gentamycin only (a2–c2) cochlear explant cultures and the compound A (a3–c3), compound B (a4–c4), and S2101 (a5–c5) pretreated cultures. The HCs were labeled with myosin VIIa antibody (green), and the nuclei were stained with DAPI (blue). Scale bar =25 μm. (C) HC counts in the apical, middle, and basal turns of the cochleae from untreated controls, gentamycin-only controls, and pretreatment with compounds A, B, and S2101. Twelve cochleae were used for each group. Data are expressed as the mean ± S.E. * p < .05 vs. the gentamycin-only group by One-Way ANOVA.